Proposal

No annual report

Introduction

Proposal ID 2015-053
Submitted January 16, 2015
Owner acsimpson
Department Forest Resources, College of
Category Machinery & Research
Funding Status Partially Funded
Metric Score 4.00

Contacts

Primary
  • Name
  • Title
  • Email
  • Phone
  • Mailbox
  • Shane Kraus
  • Sr. Computing Specialist Lead
  • skrause@uw.edu
  • 206-616-5414
  • 352100
Budget
  • Name
  • Title
  • Email
  • Phone
  • Mailbox
  • Wendy Star
  • School Administrator
  • wkstar@uw.edu
  • 206-685-2047
  • 352100
Dean
  • Name
  • Title
  • Email
  • Phone
  • Mailbox
  • Lisa Graumlich
  • Dean of College of the Environment
  • envdean@uw.edu
  • 206-221-0908
  • 352100

Descriptions

Abstract

This is a student-driven proposal to provide equipment to support the needs of student research at the School of Environmental and Forest Sciences in particular and the College of the Environment as a whole. Since the 1980’s the Forest Soils subgroup at the School of Environmental and Forest Sciences, in conjunction with the Analytical Service Center, has maintained equipment for student and lab use, including ovens, furnaces, centrifuges, pH meters, and other analytical devices. Located in the Soils Teaching Lab and ancillary unlocked rooms, this equipment is used daily by undergraduates from multiple departments working on independent research, class projects, and capstone projects, and by graduate students for their thesis and dissertation research. Much of this equipment was purchased 20-30 years ago and is now reaching the end of its lifespan. Two large pieces of equipment have broken down in the last two months and are urgently needed to complete student research projects. We propose to replace broken equipment and purchase more modern sample preparation equipment to meet the needs of the student base that uses this facility on a daily basis. In particular, we seek to purchase an EA-IRMS (Elemental Analyzer – Isotope Ratio Mass Spectrometer) that can analyze samples enriched in 13C and 15N. Stable isotope enrichment is used in a wide range of fields, from microbiology and biology to environmental engineering and ecology. To our knowledge there is not one single facility in the Pacific Northwest that is capable of analyzing 13C- or 15N- enriched solid samples; enriched samples from U.W. are shipped to Alaska, Virginia, or California. Access to this equipment – particularly, student access to this equipment – will not only raise the standard of student-available equipment from antiquated to cutting edge, but will vastly improve both the quality and potential breadth of student research.

Category Justification

This proposal falls under the category of Machine and Research in the 2014-2015 STF Request for Proposals. This proposal will fund instruments used to advance scientific research and will benefit students pursuing a range of scientific inquiry. The Soils Teaching Lab and its ancillary rooms in Bloedel Hall at SEFS are used daily by undergraduate and graduate students pursuing research projects, including dissertation research and capstone projects. This facility provides a number of pieces of equipment, including centrifuges, drying ovens and muffle furnaces, and pH meters. These are accessible to any student who needs them, regardless of department. However, most of the equipment is antiquated and recently several items have ceased to function, in particular a floor centrifuge and muffle furnace. We seek to not only replace broken equipment but expand the equipment available for student usage and provide cutting-edge equipment for studying nutrient flow in soils, vegetation, aquatic and microbial communities by purchasing an EA-IRMS.

Background

Beginning in the 1970’s and 80’s, the Forest Soils subgroup began collecting shared equipment in the Soils Teaching Lab and adjacent rooms. Equipment was purchased piecemeal from grants and made available to all students who needed it. Both graduate and undergraduate students who seek to get basic data such as moisture content and pH, prep vegetation and soil samples for elemental analysis, nutrient availability protocols, and other more complicated procedures, or perform organic matter quality and quantity analysis have come to rely on these facilities. In particular, having joint equipment under the supervision of a laboratory technician (Dongsen Xue) greatly increases undergraduate access to equipment. However, most of this equipment is now antiquated and prepares neither undergraduate nor graduate students for working in modern laboratories.

We seek to replace two recently broken pieces of equipment – our only floor centrifuge and only muffle furnace – as well as to provide three more pieces of equipment, a ball mill for quick sample preparation, a mini-centrifuge for use in DNA extractions, and an EA-IRMS (Elemental Analyzer – Isotope Ratio Mass Spectrometer) for use in not only the subject areas our students pursue - soil nutrient flow, vegetation uptake studies, and pollution research, for example – but for students in many different departments of the College of the Environment and elsewhere.

Recently a 30-year-old floor centrifuge and 50-year-old muffle furnace have reached the end of their working years. In particular, the muffle oven (used to combust soils and vegetation to analyze and quantify organic matter content, and does not require any consumables) was under constant and daily use until its demise, and there have been many complaints/queries about its absence since. We seek to replace the muffle furnace with a model equally as reliable and durable, so that we can have another 50 years of daily usage. The floor centrifuge that recently broke down was our only centrifuge powerful enough for the protocol for analyzing variable-charge soils, and is used in a number of different other analyses as well.

We also seek to provide more modern equipment for student use: a ball mill, mini-centrifuge, and an EA-IRMS (Elemental Analyzer – Isotope Ratio Mass Spectrometer). A ball mill is a standard piece of equipment in any modern soils lab and reduces weeks of grinding samples by hand to days or less of automatic sample preparation. A ball mill would also greatly reduce contamination and mess; the process of grinding by hand releases fine particles into the air. Facilities for grinding safely are available but are in a locked room in a different building which is difficult for undergraduates to gain access to.

A mini-centrifuge, in conjunction with a ball mill, would allow students to use DNA and RNA isolation kits on environmental samples without needing to gain access to a microbiology lab. Although the CGC provides equipment for post-extraction procedures such as sequencing and qPCR, there isn’t equipment available for students to actually extract DNA from their samples. Soil ecology and soil microbiology are exciting and growing fields, and we want to provide students with access to basic equipment to begin answering questions in these areas.

All of these items provide baseline sample preparation, but an EA-IRMS designed to handle samples enriched in 13C and 15N would provide unprecedented access to equipment that is generally cost-prohibitive for students. Stable isotope enrichment is a commonly used technique in many fields – microbiology, biology, aquatic sciences, ecology, and engineering, to name a few. It can be used to trace the origin of the molecules that make up our DNA, the food source of an animal, the way nutrients are cycled across a landscape of plants, the effects of pollution, or the function of a single enzyme. This is done by providing a medium or nutrient enriched in either 15N or 13C and tracing its path through a system, whether it be a cell or a landscape.

Although the IsoLab located in Earth and Space Sciences at UW and the WSU Isotope Lab are both capable of 13C and 15N analysis, neither will accept samples that have been “enriched” – that is, contain a larger-than-natural amount of either isotope. Much of the research that could be done using stable isotope analysis of enriched carbon and nitrogen is cost prohibitive not because of the cost of purchasing enriched carbon or nitrogen – which for a single student experiment may run to hundreds of dollars – but due to the cost of analysis, which can run to thousands of dollars.

An EA-IRMS, along with the above equipment to help with sample preparation, would provide the following benefits to the school:

- Provides student access to modern, cutting-edge equipment that will prepare them for working in industry, government labs, or academia
- Greatly expand the scope and breadth of undergraduate independent study and capstone projects, as well as graduate theses and dissertations
- Provide the U.W. with the only EA-IRMS to handle 13C and 15N -enriched samples in the Pacific Northwest
- Increasing intra- and inter-departmental cooperation and collaboration through shared equipment.
- Decrease the flow of grant money to outside universities for sample analysis

Benefits to Students and the University

This facility exists to provide students and labs joint access to sample preparation equipment for terrestrial environmental samples. Students can analyze samples for pH and moisture content, prepare soil and vegetation samples for elemental analysis, analyze samples for quantity and type of organic matter content, and use centrifuges for other analytical procedures such as quantifying Cation Exchange Capacity or soil nutrient content, all free of charge. With this new equipment, not only will students be able to continue these activities, but sample preparation will become much faster, more streamlined, and less likely to produce contamination. And with the addition of an EA-IRMS, U.W. students in the fields of biology, microbiology, ecology, aquatic sciences and environmental engineering will have research opportunities unavailable at any other university in the Pacific Northwest.

The hope is that with this new equipment, students will not only be able to continue to produce results but will be able to produce them faster, in greater numbers, and in fields and on subjects previously too costly for many graduate students and practically all undergraduates to pursue. Repetitive sample prep for graduate student projects - which can involve hundreds or even thousands of samples - will be greatly accelerated by purchasing a ball mill (currently all samples are ground by hand with mortar and pestle). In addition a mini-centrifuge will help undergraduate and graduate students to pursue topics in soil microbiology. An EA-IRMS, along with the above equipment to help with sample preparation, would provide the following benefits to the school:

- Provides student access to modern, cutting-edge equipment that will prepare them for working in industry, government labs, or academia
- Greatly expand the scope and breadth of undergraduate independent study and capstone projects, as well as graduate theses and dissertations
- Provide the U.W. with the only EA-IRMS to handle 13C and 15N -enriched samples in the Pacific Northwest
- Increasing intra- and inter-departmental cooperation and collaboration through shared equipment.

Departmental Endorsements

This proposal continues the School of Environmental and Forest Science’s Forest Soils subgroup’s tradition of student access to joint equipment. The SEFS IT Department will provide insurance, and Dongsen Xue, research technician for Forest Soils, in conjunction with the IT Department, will provide the equipment set-up, maintenance, and training necessary to make the facility a useful one for students. Professor David Butman, who is experienced with stable isotope equipment, will also provide oversight and maintenance.

********************
Letter from the Director:
I am writing this letter in support of Soils Group graduate students application for an equipment grant from the Student Technology Fund. I fully endorse the student’s statement of need and feel strongly that this request will have far reaching benefits to students within the School and other program’s on campus.

The infrastructure and equipment in scientific programs, such as the School of Environmental and Forest Sciences, must be updated and restored on a regular basis. The absence of such updates leads to a degradation in the quality of student experience and the ability of our faculty to adequately mentor graduate and undergraduate students in the biophysical sciences. The soils analytic laboratory in the School has experienced problems with several pieces of dated equipment and is in dire need of new and updated equipment. Items requested by the graduate students of the Soils Group include a ball mill (currently students are hand grinding samples), centrifuge, muffle oven, and CN analyzer with isotopes ratio mass spectrometer. I fully agree each of these pieces of equipment are needed in the soils laboratory either as a replacement or a new purchase.

Importantly, the soil laboratory is used by students throughout our School as well as students from other programs. The laboratory is directed by Dongsen Xue, but is specifically available for all of our students to use or work in as needed for their research programs. The soil laboratory is an amazingly important resource within the School. The Technology Fund support requested here will have a significant impact on the condition and capacity of the laboratory and benefit a large number of students.

As Director of the School, I am acutely aware of the need to maintain effective and impactful equipment and instrumentation. The equipment requested by the Soils Group graduate students will go a long ways in supporting the needs of students in the School of Environmental and Forest Sciences as well as other programs across campus. I encourage you to fully support this proposal. If you have any questions or concerns regarding this recommendation, please free to call (206-685-1928) or email (deluca@uw.edu). Thanks for your time and consideration.
Sincerely,
Thomas H. DeLuca
Professor and Director, School of Environmental and Forest Sciences
********************
13 January 2015
This letter is to support the STF Proposal entitled ‘Sample Prep and Analytical Equipment for Multi-Disciplinary Research’ submitted by the Graduate Students of the Soils Program in the School of Environmental and Forest Sciences. The joint equipment available for student use and maintained by the Forest Soils subgroup has served both undergraduate and graduate students for decades, and its update is long overdue. The equipment that the students are seeking will replace some outdated equipment, and provide new analytical capabilities for expanding research opportunities. In addition, some of the sample preparation and basic analyses are currently largely done by hand and involve extensive time for sample preparation. This student-driven proposal would enhance sample preparation capabilities, increase analytical opportunities and allow the graduate students the ability to process more samples more efficiently and to complete additional key analyses.
I strongly support their proposal and feel that it would be of benefit to not only all of the graduate students in the Soils program, but would also benefit many other students, both graduate and undergraduate who need to process samples, complete biogeochemical analyses and would like to expand on their analytical options.
Sincerely
Darlene Zabowski, PhD, Professor of Forest Soils, School of Environmental and Forest Sciences and Astrobiology

********************

I am writing in support of the request for an Elemental Analyzer-Isotope Ratio Mass Spectrometer through the STF. This instrument is vital to our studies of N-fixing organisms as 15N incorporation is the most direct quantitative assay. None of the facilities on campus with this instrument will accept labeled samples, instead limiting its use to the natural abundance differences. This restriction forces us to send our samples to other states where the wait to receive data is generally 1-3 months. Having such a long lag time between experiment and results creates major problems since there is no opportunity to adjust protocols to optimize the experiment, sometimes resulting in a full year to complete a study since we have to wait for the next growing season. If we were to have such an instrument here at UW, it would have profound impacts on our research.
Nearly a hundred undergraduate researchers have done projects in my laboratory over the years. Many of these study N-fixation but have to rely on growth measurements rather than the more direct and definitive isotope labeling studies. I also teach a Plant Microbiology Lab course for undergraduate and graduate students who would also benefit from the EA-IRMS since we use indirect assays in the course currently. Therefore, this award would substantially impact a large number of students.
Sincerely,
Sharon Doty, PhD, Associate Professor, School of Environmental and Forest Sciences and Astrobiology

********************

This letter is to support the STF Proposal entitled ‘Sample Prep and Analytical Equipment for Multi-Disciplinary Research’ submitted by the Graduate Students of the Soils Program in the School of Environmental and Forest Sciences. I can’t begin to relate how important a functioning and available analytical laboratory is to our students and research, regional, national and international. We really need to replace broken and outdated equipment, and improve the capabilities of the lab with some current equipment. Interestingly, a few years ago, when the public TV show “Dr. Science”, wanted to film a show in a dark, ancient “Alchemist”-type lab, they chose our lab in Bloedel Hall as the only suitable place on campus. That condition may be funny to many people but it shows the current state of our lab…students trying to perform miracles using outdated and sometimes barely-functioning equipment. We would still ask our students to perform miracles, but we hope to help them a bit more with a better analytical lab.
Though most of the use of this equipment is regular UW students, the scope of the work is truly international. Over the years that I have been working with this lab, we have had many students finish excellent research only possible with them working in a lab rather than just submitting samples for analysis, and receive training that helped them in future research and to secure jobs later on. The ability of Environmental Science students to do chemistry-related environmental science work is critical to their success. Also, students and faculty visiting the UW from Brazil, China, Chile, Argentina, France, Germany, Austria, Canada, Mexico and other countries have worked with our students cooperatively to generate international research. My research with the University of Sao Paulo started by bringing samples to the US nearly 30 years ago to do analyses that UNESP couldn’t do. Sadly, we’re sometimes now shipping samples to Brazil because the laboratory at the University in Brazil is better equipped than ours to do analyses we can’t do. Our lab used to be a strength for attracting students to the UW. When I show prospective grads our lab currently, they are often not too impressed. We need to change that at a world-class university like the UW.
This student-driven proposal would improve analytical opportunities and allow the students to complete analyses for their research ranging from high-school projects jointly researched with our grads, and research by our undergrads and grads. Properly equipped, the lab could also generate some funding from non-students paying for their analyses. We currently require a senior project for a degree in our school, and research projects for our graduates, and many complete at least part of that requirement in our lab. Such research supports student’s education and supports many projects to improve the environment. I strongly support their proposal and feel that it would be of benefit all UW students, not just those in our program.

Sincerely yours, Robert Harrison, PhD, Professor, School of Environmental and Forest Sciences; Affiliate Professor State University of Sao Paulo (Brazil)

********************

I want to emphasize my support for the acquisition of this EA-IRMS to be housed and managed by students within the School of Environmental and Forest Science in the College of the Environment. As properly outlined within the proposal, the application of mass-spec related science at UW is oriented around the detection of natural abundance isotope ratios. The introduction of enriched samples to the current facilities here poses significant problem to processing and equipment calibration and maintenance into the future. It is understandable why the facilities here on campus are non-responsive to running enriched 15N/13C. However, and as outlined within the proposal, the application of stable isotopes as chemical tracers in controlled experiments represents a powerful tool to understand fast and slow biogeochemical processes. The addition of this dedicated equipment would provide the ability to include tracer studies at low costs. In additional, as student enrollment within SEFS continues to increase, critical stresses on the capability of cutting edge Capstone projects to be completed already exists, placing pressure on outdated equipment. Increasing the analytical capabilities within SEFS is necessary to attract graduate students within SEFS and the College of the Environment more broadly.
I wholeheartedly support the acquisition of the EA-IRMS system. As a new faculty member, I am in the process of purchasing a Picarro Dual Isotope MS system for integrated research on carbon dioxide and methane biogeochemistry across aquatic and terrestrial ecosystems to be housed withing SEFS, and utilized with collaborators from within the School of Aquatic and Fisheries Science, and the College of Engineering. I will be overseeing that system, and I am willing to facilitate oversight and maintenance where needed on the proposed EA-IRMS.

- Dr. David Butman, Assistant Professor, School of Environmental and Forest Sciences & Civil and Environmental Engineering

Installation Timeline

If funded, all equipment excepting the EA-IRMS would be installed and available within 30 days of arrival; likely in less than a week in the case of the muffle oven. Set-up for the EA-IRMS would begin immediately but troubleshooting may take up to several months as these devices require careful calibration before functioning properly.

Resources Provided by Department

Maintenance and training for this equipment will be provided by Dongsen Xue, the primary research technician for Forest Soils in Bloedel Hall at SEFS, and Professor David Butman, who is experienced with stable isotope equipment. Dongsen Xue and Professor Darlene Zabowski routinely instruct students in how and why to use equipment and will continue to do so. Technical support is also provided by the SEFS IT Department.

Access Restrictions (if any)

Equipment in the ancillary oven room (ovens, muffle furnace, grinders etc.) is accessible to all students daily during Bloedel Hall normal business hours (M-F 7:30am – 5:15pm). Access to pH meters and centrifuges for undergraduates and non-department graduate students is arranged either through Dongsen Xue (who is available M-F 7:30am-3pm) or Professor Darlene Zabowski, for purposes of safety and training. Access for department graduate students is 24-7.

Access to the EA-IRMS would also be handled by Dongsen Xue and Professor Darlene Zabowski, who would ensure that any student using the machine is trained and supervised the first time they use the equipment.

The Soils Teaching Lab is used for classroom instruction for a total of 4-5 weeks out of the year, for hours not exceeding 15 hours per week. This room would house both the floor centrifuge and mini-centrifuge. However, because
1) the room and its equipment are used year-round for student projects, and
2) none of the equipment we are asking for will be used during classroom instruction time,
we believe that access to this equipment is well above the threshold of 50% non-instructional student access.

Student Endorsements

If this STF grant proposal were to be funded, it would greatly benefit many students in the School of Environmental and Forest Sciences (SEFS). Currently I am a graduate student in SEFS pursuing a Master’s of Science with a research interest in forest soils. In my undergraduate years studying Environmental Science and Resource Management (graduated June 2014), I had the opportunity to help with graduate research and developed my own research capstone project. Many cases, grinding and analyzing samples would take arduous long hours within a span of weeks to finish. This valuable time preparing samples could have been used more efficiently to learn and research if a ball mill and mini centrifuge for DNA extraction was readily available for use. The muffle oven and floor centrifuge that recently broke was getting very worn down until its ultimate demise. I was only able to use the muffle oven and floor centrifuge a couple times during classes and for independent projects, but it was enough to tell that the machines were limited in capability. These two pieces of equipment are heavily used daily and definitely need to be replaced soon to support undergraduate and graduate research. The last piece of equipment that I fully support to be funded is the EA-IRMS, as it would be capable of analyze enriched 15N/13C samples so that students do not have to outsource their samples to other institutions for analyzing. This would save money and would provide a unique opportunity for both undergraduate and graduate students inside and outside of our school to learn and discover more about the nutrient pathways within many different systems. All in all, this proposal if funded would be beneficial to the entire academic community here at SEFS as it will increase efficiency on data analysis, improve student preparedness for experiments, and will provide reliable equipment for everyone’s shared use.
- Lynn Khuat, 1st year Master’s student at SEFS, former ESRM undergrad

********************

To Whom it may concern,
The addition of a ball mill, mini centrifuge and a Stable Isotope Analyzer would be of great benefit of the School of Environmental and Forest Sciences and in turn the University as a whole. The mini Centrifuge is an essential tool for DNA extraction. The EA-IRNS analyzer is a game changer for the capacity of students and researchers in our department and beyond to produce high caliber publications. The ball mill would benefit all levels of soils research and analysis as ball mills turn hours of work in to mere minutes. Each of these devices has great merit to the department and the capacity of our department to support other departments at the University with their research endeavors. I strongly support their acquisition as a student and researcher with the School of Environmental and Forest Sciences at the University of Washington.

Sincerely,
Matt Norton, 2nd Year MS Student, SEFS, University of Washington

********************

As a first year PhD student, the equipment requested in this proposal would be central to the laboratory component of my dissertation. I am working to identify mechanisms of carbon storage and sequestration in soils around the Pacific Northwest. My previous experience working with equipment funded by the Student Technology Fee - namely the FTIR in Earth and Space Science and the Scanning Electron Microscope in Materials Science & Engineering - has been some of the most important training I have received in preparation for my scientific career. The EA-IRMS would be among this group in elevating my experience with modern analytical equipment, and would be a crucial part of my dissertation. Measuring 13C could only be completed on a subset of my samples and at very great expense if I have to send them to another facility for analysis. There is also a significant delay in getting data back from outside facilities, which can hinder and compress the time available for data analysis.

The muffle oven, centrifuges, and ball mill are all equipment that I would use extensively in my dissertation. I plan to complete a series of chemical dissolutions on soils from the around the Pacific Northwest which would allow me to characterize several mineralogical properties; these analyses are not possible without a working centrifuge. The ball mill would be used extensively in my work, particularly preparing samples for chemical analysis in existing UW facilities and potentially on the EA-IRMS.

As a graduate student, I rely upon help from undergraduate students for my own laboratory work and also mentor them for senior capstone projects. I have also had the chance to work with a number of undergraduate interns from universities in Brazil. The opportunity to incorporate isotope analysis into undergraduate research would substantially improve training and readiness for post-graduation work or graduate school for all of these students. I am presently working with a student who would be using the EA-IRMS if it were here now, and I have little doubt that this would generate substantial interest from future undergraduate students. I think it is extremely important that this proposal be funded, both for my own research and for the education of future undergraduate and graduate students.

- Jason James, 1st year PhD Student, SEFS, University of Washington

********************

As a PhD student studying carbon cycling in soils, a number of these pieces of equipment will be invaluable to my research. I am currently investigating the effects of variable charge minerals on soil carbon storage, but have been unable to analyze the anion exchange capacity of my samples without a properly working centrifuge and will not be able to complete this project until I gain access to one. Other research that I am involved in at the same site will require grinding litter samples in order to quantify total carbon throughout the soil profile. This process will be made much more efficient by access to a ball mill. The three undergraduates working on this project will also benefit from this more efficient process by being able to delve deeper into the research. As the use of enriched 13C to study soil carbon cycling was previously cost prohibitive, access to an EA-IRM has the potential to significantly broaden the scope of my research and will allow me to develop additional skills that will prove useful in future research.

- Christiana Dietzen, 2nd year PhD student, SEFS, University of Washington
********************

As an undergraduate of the Earth and Space Science Department, I have been fortunate enough to begin research in my minor's department: the School of Environmental and Forest Resources. Coming from the ESS department, the chance to do research in SEFS is the perfect complement to my studies. It not only exposes me to the process of working in a lab but also provides insight into the field of environmental science that I wouldn't otherwise gain in ESS. In addition, this research has allowed me to pursue honors within the ESS department.
Graduate student mentorship has been instrumental in both providing this opportunity to test carbon to nitrogen ratios in plant tissues as well as demonstrating the process by which this is done. Part of this process requires extensive grinding of plant roots and leaves. While the use of a mortar and pestle proves effective, it does not prove efficient. This method requires time-consuming washing and drying of the mortar and pestle as well as pain staking manual grinding. For the sake of future students' time (and wrist muscles), and in hopes of gathering more consistent and timely results, a ball mill would prove invaluable. Furthermore, a muffle furnace, which was essential to my introduction to soils course and class project, absolutely needs replacement. After 50 years of service, the investment in this furnace has proven worthwhile, but the investment needs to be made again for both educational and research purposes. Personally, I wish these tools were available to me now, during my time of research. That being said, I believe the best decision, in this case, is to make this equipment available not only to graduate researchers, but also to the undergraduate students who will come after me.
- Susan Wiseman, 4th Year Honor’s Student, ESS
********************

The acquisition of updated laboratory equipment and analytical tools will immensely enhance the quality of education, research, teaching at the School of Environmental and Forest Sciences and, in particular, the Forest Soils subgroup of which I am part of. On many occasions my activities as both a student and researcher were halted because the equipment in our shared facility was broken or out of order. Over the summer, I collected time-sensitive soil samples that required immediate extraction and filtration. A new floor centrifuge and muffle oven would have allowed me to process more samples at once and my data would reflect a closer estimate of values that better represent the characteristics of soils in the field. I have also had undergraduate students use the muffle oven and ball mill. Their hard work would have been less time consuming and distracting from the main research objectives if the equipment was working efficiently. The additional items, such as the DNA extraction kit and EA-IRMS, would transport this lab group into this century and perhaps the next. As a group, we strive to think ahead of current research, develop new techniques, and create unique perspectives of our research and educational topics. The equipment purposed for the STF grand would facilitate an advanced and independent research and education environment that meets the mission of this university – to preserve, advance, and disseminate knowledge. Thank you for your time and consideration.
- Melissa R. Pingree, 2nd-year PhD Student, SEFS

********************

I strongly support the acquisition of this equipment. It is extremely important for the continuity and quality of the research conducted by undergraduate and graduate students at the School of Environmental and Forest Sciences as well as the College of the Environment. Moreover, this will give students the opportunity to work with high quality and precision equipment, allowing them to learn more advanced techniques of sample preparation and analysis.
The equipment available in the teaching lab has been intensively used by undergraduate and graduate students on a daily basis to conduct analysis related to their independent studies, theses and dissertations. The data obtained have resulted in high quality scientific papers published in relevant journals and scientific magazines.
Besides that, the acquisition of the EA-DRMS equipment to handle 13C and 15N-enriched samples will help to improve the quality of the researches conducted by the students. This can be invested in other ways, such as improvement of the teaching lab installations and adjacent areas, as well as acquisition of equipment and materials for research use in the department.
- Marcella Menegale, 3rd –year PhD Student, SEFS

********************

My dissertation research seeks to understand the movement of applied nitrogen fertilizers within Douglas-fir forests of the Pacific Northwest. The fertilizers were enriched to 0.5 atom% 15N (~370 0/00 15N). Using a fertilizer enriched with 15N, I have been able to trace the applied fertilizer as it moves between N pools within the study area. After the samples are processed here at the University of Washington, they are sent to Virginia Tech for analysis using their isotope ratio mass spectrometer (IRMS). Their graduate and undergraduate students run our samples and it takes months to receive the data back. Additionally, our undergraduate research assistants do not benefit from the experiential learning opportunity with this increasingly important analytical equipment. We have employed the help of 8 undergraduates on this project alone; however, the scope of their involvement has been limited due to a lack of access to equipment. Currently, the weighing of samples and use of the isotope facilities in Earth and Space Sciences has been limited to our graduate students because of the concern for contaminating their samples with our heavily enriched samples. Having access to an EA-IRMS within the School of Environmental and Forest Sciences would also expand on the opportunities our undergraduates have for their own research projects and methods that can be taught in our courses. Having to send samples to other universities for analysis makes it impractical to use these methods in the lab portion of our courses or for the application in a capstone project because the turn-around time for outside analysis has been on the order of months. Training with modern, functioning equipment is important for the efficient completion of our research projects and as preparation for employment in environmental science fields where expertise with these tools is commonplace.
- Stephani Michelsen-Correa, 3rd-year PhD Student, SEFS

********************

I am a PhD student in the School of Environmental and Forest Sciences, studying plant-microbe symbiotic relationships. I specifically use diazotrophic (N-fixing) endophytes to inoculate rice and corn plants to investigate the symbiotic relationship between endophytes and host plants. They fix atmospheric nitrogen and make it available to the host plants to utilize. An EA-IRMS to analyze for 15N/13C in enriched plant samples is really useful for my research. In fact, currently I am sending my plant samples in Alaska to analyze, and they cost about $17.00 per sample. I am writing in support of the application acquiring EA-IRMS in our school. The acquisition of these instruments in our school would directly benefit my research, and save lots of money too. I eagerly look forward to having more of these instruments in our school so that they are always available when needed.

-Shyam Kandel, Graduate Student, School of Environmental and Forest Sciences

Items

Group Funded Item Unit price Quantity Subtotal
Ball Mill

Mixer Mill MM 200 (MM 200 100–240 V, 50/60 Hz)

$6,840.00 1 $6,840.00
Description

http://www.retsch.com/products/milling/ball-mills/mixer-mill-mm-200/
The mixer mill MM 200 is a compact versatile benchtop unit, which has been developed specially for dry grinding of small amounts of sample. It can mix and homogenize powders in only a few seconds. It is also perfectly suitable for the disruption of biological cells as well as for DNA/RNA extraction. With its high performance and great flexibility the MM 200 is a unique product in the market.

- reproducible, efficient grinding, mixing and homogenization in seconds
-powerful grinding by impact and friction, up to 25 Hz for up to 20 samples per run
-9 SOPs can be stored
-wide range of accessories including various jar and ball sizes, adapter racks, grinding tool materials

Justification

A ball mill is a standard piece of equipment in any modern lab that deals with soil and plant tissue samples, and reduces weeks of grinding samples by hand to days or less of automatic sample preparation. A ball mill would also greatly reduce contamination and mess; the process of grinding by hand releases fine particles into the air. Facilities for grinding safely are available but are in a locked room in a different building which is difficult for undergraduates to gain access to. This ball mill would also be of great help in preparing soil and plant tissue samples for DNA extraction. Currently mortar and pestle must be autoclaved and frozen at -80 degrees C, and the tissue ground with liquid nitrogen. Preparing a single DNA extraction may take more than 2 hours. A ball mill would reduce this time to minutes.

This particular grinder is ideal because it is small and can fit on a countertop, and so can be located in the oven room which is accessible to all students.

Stainless Steel 1.5ml grinding jar for ball mill

$391.00 1 $391.00
Description

An accessory for the ball mill for grinding small samples of less than 1ml.

Justification

A grinding jar is a necessary accessory for the ball mill - it is where the actual grinding takes place.

Stainless Steel 10ml Grinding Jar for Ball Mill

$443.00 1 $443.00
Description

An accessory for the ball mill for grinding small samples of about 5ml (jar can only be half-full of sample).

Justification

A grinding jar is a necessary accessory for the ball mill - it is where the actual grinding takes place.

Mini-Centrifuge

Gusto High Speed Mini-Centrifuge

$975.00 1 $975.00
Description

http://www.rpicorp.com/products/prod_info.html?products_front_id=2252&catid=5&cat_id=61

"This compact centrifuge is ideal for most protocols calling for fast spins (up to 12,500 rpm / 9,800 rcf) and quick spin-downs. Useful for PCR, microfilter cell separation, clinical applications, and HPLC protocols. Operation is extremely simple for this high-level of sophistication: Insert tubes, close lid, adjust speed/time, and press Start. For duplicate spins, simply reload rotor and push Start. Designed with quick-spin option for commonly used 20-second spins. Also includes a tool-free, quick-change rotor system.

Safety features ensure the unit will not operate with an open lid, a jammed rotor or when out of balance. Gusto provides the option of setting and displaying in RPM or RCF. Efficiently designed for minimum of bench top space. Included features typically found on more expensive units.

Tested to comply with U.S., Canadian and European Safety and EMC standards.
Product Specifications:

Speed: 500 - 12,500 RPM
RCF: 15 - 9,800 x g
Dimensions (mm): 203 x 171 x 114
Includes two rotors:
1 - 12 x 1.5-2.0ml rotor
1 - 32 x 0.2ml PCR rotor
Adapters for 0.2-0.5ml tubes
Power: 100 - 240 VAC, 50/60 with four interchangeable power cords for U.S./Japan/Taiwan, Continental Europe/Israel, Australia and United Kingdom
Warranty: 2 Years"

Justification

A mini-centrifuge, in conjunction with a ball mill, would allow students to use DNA and RNA isolation kits on environmental samples without needing to gain access to a microbiology lab. Although the CGC provides equipment for post-extraction procedures such as sequencing and qPCR, there isn’t equipment available for students to actually extract DNA from their samples. Soil ecology and soil microbiology are exciting and growing fields, and we want to provide students with access to basic equipment to begin answering questions in these areas.

This particular model is much smaller and cheaper than most mini-centrifuges but still capable of the speeds and times necessary for DNA extraction procedures.

Muffle Furnace

Muffle Furnace

$8,849.00 1 $8,849.00
Description

http://www.thermoscientific.com/en/product/lindberg-blue-m-lgo-box-furnaces.html

Lindberg/Blue M™ LGO Box Furnaces

Utilize the latest technical advances in heating elements, insulation and temperature control integrated into a self-contained cabinet with Thermo Scientific™ Lindberg/Blue M™ LGO Box Furnaces. Furnaces feature proprietary LGO (light gauge overbend) heating elements and Thermo Scientific™ Moldatherm™ insulation for efficient and economical transfer of heat to chamber, with low exterior temperatures. Variable heat-up rate eliminates thermal shock to materials with inherently quick heat up and cool down rates.
- See more at: http://www.thermoscientific.com/content/tfs/en/product/lindberg-blue-m-lgo-box-furnaces.html#sthash.EGjhMZzN.dpuf

Justification

Recently a 50-year-old muffle furnace have reached the end of their working years. In particular, the muffle oven (used to combust soils and vegetation to analyze and quantify organic matter content, and does not require any consumables) was under constant and daily use until its demise, and there have been many complaints/queries about its absence since. We seek to replace the muffle furnace with a model equally as reliable and durable, so that we can have another 50 years of daily usage. This model is durable enough to withstand another 50 years.

Floor Centrifuge

Thermo Scientific Heraeus Multifuge X3F Floor Centrifuge

$9,098.00 1 $9,098.00
Description

The Heraeus Multifuge X3F centrifuge allows you to accelerate your research by processing up to 40 x 50 mL conical tubes (100% more) in your swing-out rotor, or up to 14 x 50 mL (133% more) in fixed angle configuration, for increased capacity over competitive models in the same bench space.
The floor-standing configuration conserves valuable bench space and, with a smaller footpint than a rolling cart, saves additional lab space.
With locking casters, this centrifuge is easily transported, positioned, and safely locked for enhanced flexibility and improved ergonomics during loading, unloading, and everyday programming.

Justification

Recently a 30-year-old floor centrifuge a reached the end of its working years. The floor centrifuge that recently broke down was our only centrifuge powerful enough for the protocol for analyzing variable-charge soils, and is used in a number of different other analyses as well.

EA-IRMS

New Generation Integra2 combined IRMS and elemental analyser

$103,700.00 0 $0.00
Description

The Integra2 is a combination of Sercon's 20-22 mass spectrometer and the SL elemental analyser. This unique product provides the isotope researcher with the most compact instrument. Capable of analysing samples for both 15N and 13C simultaneously with an 18O option, the Integra2 provides the most cost effective and easy to use isotopic analyser available.

The Integra2 is the instrument of choice for both natural abundance and enriched sample analysis.

The space saving Integra series were designed to be the workhorses for 13C and 15N analysis. These instruments have a pedigree that goes back to the world’s first bench-top isotope ratio mass spectrometer that was introduced by Europa Scientific in 1986. These instruments provide simplicity of operation, ease of maintenance and performance on a budget.

Based on the high performance 20-22 stable isotope analyser and SL sample preparation module the Integra2 is a fully integrated combustion, purification and measurement system for 15N and 13C.

Justification

(Sercon is based in the UK - price includes tariffs from Canada, where product ships from)

but an EA-IRMS designed to handle samples enriched in 13C and 15N would provide unprecedented access to equipment that is generally cost-prohibitive for students. Stable isotope enrichment is a commonly used technique in many fields – microbiology, biology, aquatic sciences, ecology, and engineering, to name a few. It can be used to trace the origin of the molecules that make up our DNA, the food source of an animal, the way nutrients are cycled across a landscape of plants, the effects of pollution, or the function of a single enzyme. This is done by providing a medium or nutrient enriched in either 15N or 13C and tracing its path through a system, whether it be a cell or a landscape.
Although the IsoLab located in Earth and Space Sciences at UW and the WSU Isotope Lab are both capable of 13C and 15N analysis, neither will accept samples that have been “enriched” – that is, contain a larger-than-natural amount of either isotope. Much of the research that could be done using stable isotope analysis of enriched carbon and nitrogen is cost prohibitive not because of the cost of purchasing enriched carbon or nitrogen – which for a single student experiment may run to hundreds of dollars – but due to the cost of analysis, which can run to thousands of dollars.
An EA-IRMS, along with the above equipment to help with sample preparation, would provide the following benefits to the school:
- Provides student access to modern, cutting-edge equipment that will prepare them for working in industry, government labs, or academia
- Greatly expand the scope and breadth of undergraduate independent study and capstone projects, as well as graduate theses and dissertations
- Provide the U.W. with the only EA-IRMS to handle 13C and 15N -enriched samples in the Pacific Northwest
- Increasing intra- and inter-departmental cooperation and collaboration through shared equipment.
- Decrease the flow of grant money to outside universities for sample analysis

Total requested: $130,296.00

Total funded: $26,596.00

Group Funded Item Change in Unit price Change in Quantity Change in Subtotal
Ball Mill

Mixer Mill MM 200 (MM 200 100–240 V, 50/60 Hz)

-$342.00 0 -$342.00
Description

http://www.retsch.com/products/milling/ball-mills/mixer-mill-mm-200/
The mixer mill MM 200 is a compact versatile benchtop unit, which has been developed specially for dry grinding of small amounts of sample. It can mix and homogenize powders in only a few seconds. It is also perfectly suitable for the disruption of biological cells as well as for DNA/RNA extraction. With its high performance and great flexibility the MM 200 is a unique product in the market.

- reproducible, efficient grinding, mixing and homogenization in seconds
-powerful grinding by impact and friction, up to 25 Hz for up to 20 samples per run
-9 SOPs can be stored
-wide range of accessories including various jar and ball sizes, adapter racks, grinding tool materials

Justification

A ball mill is a standard piece of equipment in any modern lab that deals with soil and plant tissue samples, and reduces weeks of grinding samples by hand to days or less of automatic sample preparation. A ball mill would also greatly reduce contamination and mess; the process of grinding by hand releases fine particles into the air. Facilities for grinding safely are available but are in a locked room in a different building which is difficult for undergraduates to gain access to. This ball mill would also be of great help in preparing soil and plant tissue samples for DNA extraction. Currently mortar and pestle must be autoclaved and frozen at -80 degrees C, and the tissue ground with liquid nitrogen. Preparing a single DNA extraction may take more than 2 hours. A ball mill would reduce this time to minutes.

This particular grinder is ideal because it is small and can fit on a countertop, and so can be located in the oven room which is accessible to all students.

Stainless Steel 1.5ml grinding jar for ball mill

-$391.00 -1 -$391.00
Description

An accessory for the ball mill for grinding small samples of less than 1ml.

Justification

A grinding jar is a necessary accessory for the ball mill - it is where the actual grinding takes place.

Stainless Steel 10ml Grinding Jar for Ball Mill

-$22.15 1 $398.70
Description

An accessory for the ball mill for grinding small samples of about 5ml (jar can only be half-full of sample).

Justification

A grinding jar is a necessary accessory for the ball mill - it is where the actual grinding takes place.

ADAPTERRACK , MADE OF PTFE, FOR 5 VIALS 1.5 ML AND 2.0 ML

$375.25 2 $750.50
Description

Ball mill adapter for five 1.5 or 2 ml grinding tubes. Thus 10 1.5 or 2ml tubes can be ground at once.

Justification

Rather than purchase a 1.5ml grinding jar, this adapter allows for multiple 1.5 or 2ml containers to be used at once for the same price. This also means it can be used in place of a PowerLyzer or FastPrep machine in DNA extraction.

Why two of them? This ball mill has two slots for jars or adapters for grinding, and I have been told by the company sales rep that the mill will not operate unless both slots are filled with the same type of jar or adapter (hence the purchase of 2 of the 10ml jars as well).

GRINDING BALL STAINLESS STEEL 10MM Ø

$3.42 4 $13.68
Description

10mm steel grinding balls for the 10ml grinding jars

Justification

These are the appropriate-sized grinding balls for the 10ml jar; since they are only $13 total I thought to include them here.

Mini-Centrifuge

Gusto High Speed Mini-Centrifuge

-$975.00 -1 -$975.00
Description

http://www.rpicorp.com/products/prod_info.html?products_front_id=2252&catid=5&cat_id=61

"This compact centrifuge is ideal for most protocols calling for fast spins (up to 12,500 rpm / 9,800 rcf) and quick spin-downs. Useful for PCR, microfilter cell separation, clinical applications, and HPLC protocols. Operation is extremely simple for this high-level of sophistication: Insert tubes, close lid, adjust speed/time, and press Start. For duplicate spins, simply reload rotor and push Start. Designed with quick-spin option for commonly used 20-second spins. Also includes a tool-free, quick-change rotor system.

Safety features ensure the unit will not operate with an open lid, a jammed rotor or when out of balance. Gusto provides the option of setting and displaying in RPM or RCF. Efficiently designed for minimum of bench top space. Included features typically found on more expensive units.

Tested to comply with U.S., Canadian and European Safety and EMC standards.
Product Specifications:

Speed: 500 - 12,500 RPM
RCF: 15 - 9,800 x g
Dimensions (mm): 203 x 171 x 114
Includes two rotors:
1 - 12 x 1.5-2.0ml rotor
1 - 32 x 0.2ml PCR rotor
Adapters for 0.2-0.5ml tubes
Power: 100 - 240 VAC, 50/60 with four interchangeable power cords for U.S./Japan/Taiwan, Continental Europe/Israel, Australia and United Kingdom
Warranty: 2 Years"

Justification

A mini-centrifuge, in conjunction with a ball mill, would allow students to use DNA and RNA isolation kits on environmental samples without needing to gain access to a microbiology lab. Although the CGC provides equipment for post-extraction procedures such as sequencing and qPCR, there isn’t equipment available for students to actually extract DNA from their samples. Soil ecology and soil microbiology are exciting and growing fields, and we want to provide students with access to basic equipment to begin answering questions in these areas.

This particular model is much smaller and cheaper than most mini-centrifuges but still capable of the speeds and times necessary for DNA extraction procedures.

Muffle Furnace

Muffle Furnace

-$1,507.37 0 -$1,507.37
Description

http://www.thermoscientific.com/en/product/lindberg-blue-m-lgo-box-furnaces.html

Lindberg/Blue M™ LGO Box Furnaces

Utilize the latest technical advances in heating elements, insulation and temperature control integrated into a self-contained cabinet with Thermo Scientific™ Lindberg/Blue M™ LGO Box Furnaces. Furnaces feature proprietary LGO (light gauge overbend) heating elements and Thermo Scientific™ Moldatherm™ insulation for efficient and economical transfer of heat to chamber, with low exterior temperatures. Variable heat-up rate eliminates thermal shock to materials with inherently quick heat up and cool down rates.
- See more at: http://www.thermoscientific.com/content/tfs/en/product/lindberg-blue-m-lgo-box-furnaces.html#sthash.EGjhMZzN.dpuf

Justification

Recently a 50-year-old muffle furnace have reached the end of their working years. In particular, the muffle oven (used to combust soils and vegetation to analyze and quantify organic matter content, and does not require any consumables) was under constant and daily use until its demise, and there have been many complaints/queries about its absence since. We seek to replace the muffle furnace with a model equally as reliable and durable, so that we can have another 50 years of daily usage. This model is durable enough to withstand another 50 years.

Floor Centrifuge

Thermo Scientific Heraeus Multifuge X3F Floor Centrifuge

-$9,098.00 -1 -$9,098.00
Description

The Heraeus Multifuge X3F centrifuge allows you to accelerate your research by processing up to 40 x 50 mL conical tubes (100% more) in your swing-out rotor, or up to 14 x 50 mL (133% more) in fixed angle configuration, for increased capacity over competitive models in the same bench space.
The floor-standing configuration conserves valuable bench space and, with a smaller footpint than a rolling cart, saves additional lab space.
With locking casters, this centrifuge is easily transported, positioned, and safely locked for enhanced flexibility and improved ergonomics during loading, unloading, and everyday programming.

Justification

Recently a 30-year-old floor centrifuge a reached the end of its working years. The floor centrifuge that recently broke down was our only centrifuge powerful enough for the protocol for analyzing variable-charge soils, and is used in a number of different other analyses as well.

Centrifuges

Floor and Mini Centrifuges from Beckman

$14,000.00 1 $14,000.00
Description

Micro-centrifuge:
https://www.beckmancoulter.com/wsrportal/wsrportal.portal?_nfpb=true&_windowLabel=UCM_RENDERER&_urlType=render&wlpUCM_RENDERER_path=%2Fwsr%2Fresearch-and-discovery%2Fproducts-and-services%2Fcentrifugation%2Fmicrofuge-16%2Findex.htm#2/10//0/25/1/0/asc/2/A46472///0/1//0/%2Fwsrportal%2Fwsr%2Fresearch-and-discovery%2Fproducts-and-services%2Fcentrifugation%2Fmicrofuge-16%2Findex.htm/

This is a compact micro-centrifuge that definitely provides the speeds students would need to use DNA extraction kits. The nice feature about this centrifuge (as compared to the Gusto) us that the rotor can be removed and autoclaved.

Avanti-JE Floor Centrifuge:
https://www.beckmancoulter.com/wsrportal/wsrportal/wsr/industrial/products/centrifugation/avanti-j-e/index.htm?i=369001#2/10//0/25/1/0/asc/2/369001///0/1//0/%2Fwsrportal%2Fwsr%2Findustrial%2Fproducts%2Fcentrifugation%2Favanti-j-e%2Findex.htm/

This centrifuge is the very-much-updated version of the centrifuge we have now, and is thus compatible with the rotors we have now. In addition it tests each rotor at low speed before beginning a run and will correct the parameters for the run if they've been entered incorrectly (a nice feature to have for a centrifuge being used by many students...)

Justification

Unfortunately the original floor centrifuge we had included is not compatible with our rotors (which generally cost about a thousand dollars each). Beckman has provided a very generous 50% discount on a new centrifuge that would be compatible with the rotors, and are willing to throw in a micro-centrifuge as well. We realize this is in excess of the amount we asked for, and if not approved we can look for a used floor centrifuge that is compatible with our rotors, but this is such a great discount we thought we'd ask!

Supplemental request: $2,849.51

Deicision: Funded

Group Funded Item Unit price Quantity Subtotal
Ball Mill

Mixer Mill MM 200 (MM 200 100–240 V, 50/60 Hz)

$6,498.00 1 $6,498.00
Description

http://www.retsch.com/products/milling/ball-mills/mixer-mill-mm-200/
The mixer mill MM 200 is a compact versatile benchtop unit, which has been developed specially for dry grinding of small amounts of sample. It can mix and homogenize powders in only a few seconds. It is also perfectly suitable for the disruption of biological cells as well as for DNA/RNA extraction. With its high performance and great flexibility the MM 200 is a unique product in the market.

- reproducible, efficient grinding, mixing and homogenization in seconds
-powerful grinding by impact and friction, up to 25 Hz for up to 20 samples per run
-9 SOPs can be stored
-wide range of accessories including various jar and ball sizes, adapter racks, grinding tool materials

Justification

A ball mill is a standard piece of equipment in any modern lab that deals with soil and plant tissue samples, and reduces weeks of grinding samples by hand to days or less of automatic sample preparation. A ball mill would also greatly reduce contamination and mess; the process of grinding by hand releases fine particles into the air. Facilities for grinding safely are available but are in a locked room in a different building which is difficult for undergraduates to gain access to. This ball mill would also be of great help in preparing soil and plant tissue samples for DNA extraction. Currently mortar and pestle must be autoclaved and frozen at -80 degrees C, and the tissue ground with liquid nitrogen. Preparing a single DNA extraction may take more than 2 hours. A ball mill would reduce this time to minutes.

This particular grinder is ideal because it is small and can fit on a countertop, and so can be located in the oven room which is accessible to all students.

Stainless Steel 1.5ml grinding jar for ball mill

$0.00 0 $0.00
Description

An accessory for the ball mill for grinding small samples of less than 1ml.

Justification

A grinding jar is a necessary accessory for the ball mill - it is where the actual grinding takes place.

Stainless Steel 10ml Grinding Jar for Ball Mill

$420.85 2 $841.70
Description

An accessory for the ball mill for grinding small samples of about 5ml (jar can only be half-full of sample).

Justification

A grinding jar is a necessary accessory for the ball mill - it is where the actual grinding takes place.

ADAPTERRACK , MADE OF PTFE, FOR 5 VIALS 1.5 ML AND 2.0 ML

$375.25 2 $750.50
Description

Ball mill adapter for five 1.5 or 2 ml grinding tubes. Thus 10 1.5 or 2ml tubes can be ground at once.

Justification

Rather than purchase a 1.5ml grinding jar, this adapter allows for multiple 1.5 or 2ml containers to be used at once for the same price. This also means it can be used in place of a PowerLyzer or FastPrep machine in DNA extraction.

Why two of them? This ball mill has two slots for jars or adapters for grinding, and I have been told by the company sales rep that the mill will not operate unless both slots are filled with the same type of jar or adapter (hence the purchase of 2 of the 10ml jars as well).

GRINDING BALL STAINLESS STEEL 10MM Ø

$3.42 4 $13.68
Description

10mm steel grinding balls for the 10ml grinding jars

Justification

These are the appropriate-sized grinding balls for the 10ml jar; since they are only $13 total I thought to include them here.

Mini-Centrifuge

Gusto High Speed Mini-Centrifuge

$0.00 0 $0.00
Description

http://www.rpicorp.com/products/prod_info.html?products_front_id=2252&catid=5&cat_id=61

"This compact centrifuge is ideal for most protocols calling for fast spins (up to 12,500 rpm / 9,800 rcf) and quick spin-downs. Useful for PCR, microfilter cell separation, clinical applications, and HPLC protocols. Operation is extremely simple for this high-level of sophistication: Insert tubes, close lid, adjust speed/time, and press Start. For duplicate spins, simply reload rotor and push Start. Designed with quick-spin option for commonly used 20-second spins. Also includes a tool-free, quick-change rotor system.

Safety features ensure the unit will not operate with an open lid, a jammed rotor or when out of balance. Gusto provides the option of setting and displaying in RPM or RCF. Efficiently designed for minimum of bench top space. Included features typically found on more expensive units.

Tested to comply with U.S., Canadian and European Safety and EMC standards.
Product Specifications:

Speed: 500 - 12,500 RPM
RCF: 15 - 9,800 x g
Dimensions (mm): 203 x 171 x 114
Includes two rotors:
1 - 12 x 1.5-2.0ml rotor
1 - 32 x 0.2ml PCR rotor
Adapters for 0.2-0.5ml tubes
Power: 100 - 240 VAC, 50/60 with four interchangeable power cords for U.S./Japan/Taiwan, Continental Europe/Israel, Australia and United Kingdom
Warranty: 2 Years"

Justification

A mini-centrifuge, in conjunction with a ball mill, would allow students to use DNA and RNA isolation kits on environmental samples without needing to gain access to a microbiology lab. Although the CGC provides equipment for post-extraction procedures such as sequencing and qPCR, there isn’t equipment available for students to actually extract DNA from their samples. Soil ecology and soil microbiology are exciting and growing fields, and we want to provide students with access to basic equipment to begin answering questions in these areas.

This particular model is much smaller and cheaper than most mini-centrifuges but still capable of the speeds and times necessary for DNA extraction procedures.

Muffle Furnace

Muffle Furnace

$7,341.63 1 $7,341.63
Description

http://www.thermoscientific.com/en/product/lindberg-blue-m-lgo-box-furnaces.html

Lindberg/Blue M™ LGO Box Furnaces

Utilize the latest technical advances in heating elements, insulation and temperature control integrated into a self-contained cabinet with Thermo Scientific™ Lindberg/Blue M™ LGO Box Furnaces. Furnaces feature proprietary LGO (light gauge overbend) heating elements and Thermo Scientific™ Moldatherm™ insulation for efficient and economical transfer of heat to chamber, with low exterior temperatures. Variable heat-up rate eliminates thermal shock to materials with inherently quick heat up and cool down rates.
- See more at: http://www.thermoscientific.com/content/tfs/en/product/lindberg-blue-m-lgo-box-furnaces.html#sthash.EGjhMZzN.dpuf

Justification

Recently a 50-year-old muffle furnace have reached the end of their working years. In particular, the muffle oven (used to combust soils and vegetation to analyze and quantify organic matter content, and does not require any consumables) was under constant and daily use until its demise, and there have been many complaints/queries about its absence since. We seek to replace the muffle furnace with a model equally as reliable and durable, so that we can have another 50 years of daily usage. This model is durable enough to withstand another 50 years.

Floor Centrifuge

Thermo Scientific Heraeus Multifuge X3F Floor Centrifuge

$0.00 0 $0.00
Description

The Heraeus Multifuge X3F centrifuge allows you to accelerate your research by processing up to 40 x 50 mL conical tubes (100% more) in your swing-out rotor, or up to 14 x 50 mL (133% more) in fixed angle configuration, for increased capacity over competitive models in the same bench space.
The floor-standing configuration conserves valuable bench space and, with a smaller footpint than a rolling cart, saves additional lab space.
With locking casters, this centrifuge is easily transported, positioned, and safely locked for enhanced flexibility and improved ergonomics during loading, unloading, and everyday programming.

Justification

Recently a 30-year-old floor centrifuge a reached the end of its working years. The floor centrifuge that recently broke down was our only centrifuge powerful enough for the protocol for analyzing variable-charge soils, and is used in a number of different other analyses as well.

EA-IRMS

New Generation Integra2 combined IRMS and elemental analyser

$103,700.00 0 $0.00
Description

The Integra2 is a combination of Sercon's 20-22 mass spectrometer and the SL elemental analyser. This unique product provides the isotope researcher with the most compact instrument. Capable of analysing samples for both 15N and 13C simultaneously with an 18O option, the Integra2 provides the most cost effective and easy to use isotopic analyser available.

The Integra2 is the instrument of choice for both natural abundance and enriched sample analysis.

The space saving Integra series were designed to be the workhorses for 13C and 15N analysis. These instruments have a pedigree that goes back to the world’s first bench-top isotope ratio mass spectrometer that was introduced by Europa Scientific in 1986. These instruments provide simplicity of operation, ease of maintenance and performance on a budget.

Based on the high performance 20-22 stable isotope analyser and SL sample preparation module the Integra2 is a fully integrated combustion, purification and measurement system for 15N and 13C.

Justification

(Sercon is based in the UK - price includes tariffs from Canada, where product ships from)

but an EA-IRMS designed to handle samples enriched in 13C and 15N would provide unprecedented access to equipment that is generally cost-prohibitive for students. Stable isotope enrichment is a commonly used technique in many fields – microbiology, biology, aquatic sciences, ecology, and engineering, to name a few. It can be used to trace the origin of the molecules that make up our DNA, the food source of an animal, the way nutrients are cycled across a landscape of plants, the effects of pollution, or the function of a single enzyme. This is done by providing a medium or nutrient enriched in either 15N or 13C and tracing its path through a system, whether it be a cell or a landscape.
Although the IsoLab located in Earth and Space Sciences at UW and the WSU Isotope Lab are both capable of 13C and 15N analysis, neither will accept samples that have been “enriched” – that is, contain a larger-than-natural amount of either isotope. Much of the research that could be done using stable isotope analysis of enriched carbon and nitrogen is cost prohibitive not because of the cost of purchasing enriched carbon or nitrogen – which for a single student experiment may run to hundreds of dollars – but due to the cost of analysis, which can run to thousands of dollars.
An EA-IRMS, along with the above equipment to help with sample preparation, would provide the following benefits to the school:
- Provides student access to modern, cutting-edge equipment that will prepare them for working in industry, government labs, or academia
- Greatly expand the scope and breadth of undergraduate independent study and capstone projects, as well as graduate theses and dissertations
- Provide the U.W. with the only EA-IRMS to handle 13C and 15N -enriched samples in the Pacific Northwest
- Increasing intra- and inter-departmental cooperation and collaboration through shared equipment.
- Decrease the flow of grant money to outside universities for sample analysis

Centrifuges

Floor and Mini Centrifuges from Beckman

$14,000.00 1 $14,000.00
Description

Micro-centrifuge:
https://www.beckmancoulter.com/wsrportal/wsrportal.portal?_nfpb=true&_windowLabel=UCM_RENDERER&_urlType=render&wlpUCM_RENDERER_path=%2Fwsr%2Fresearch-and-discovery%2Fproducts-and-services%2Fcentrifugation%2Fmicrofuge-16%2Findex.htm#2/10//0/25/1/0/asc/2/A46472///0/1//0/%2Fwsrportal%2Fwsr%2Fresearch-and-discovery%2Fproducts-and-services%2Fcentrifugation%2Fmicrofuge-16%2Findex.htm/

This is a compact micro-centrifuge that definitely provides the speeds students would need to use DNA extraction kits. The nice feature about this centrifuge (as compared to the Gusto) us that the rotor can be removed and autoclaved.

Avanti-JE Floor Centrifuge:
https://www.beckmancoulter.com/wsrportal/wsrportal/wsr/industrial/products/centrifugation/avanti-j-e/index.htm?i=369001#2/10//0/25/1/0/asc/2/369001///0/1//0/%2Fwsrportal%2Fwsr%2Findustrial%2Fproducts%2Fcentrifugation%2Favanti-j-e%2Findex.htm/

This centrifuge is the very-much-updated version of the centrifuge we have now, and is thus compatible with the rotors we have now. In addition it tests each rotor at low speed before beginning a run and will correct the parameters for the run if they've been entered incorrectly (a nice feature to have for a centrifuge being used by many students...)

Justification

Unfortunately the original floor centrifuge we had included is not compatible with our rotors (which generally cost about a thousand dollars each). Beckman has provided a very generous 50% discount on a new centrifuge that would be compatible with the rotors, and are willing to throw in a micro-centrifuge as well. We realize this is in excess of the amount we asked for, and if not approved we can look for a used floor centrifuge that is compatible with our rotors, but this is such a great discount we thought we'd ask!

Overall total funded: $29,445.51

Comments

No comments